TNFα and RANKL stimulation regulates the expression of p21 in <i>Pkd1</i> wild type MEK cells.

<p>(A) Western blot analysis of the expression of Id2 and p21 from whole cell lysates of <i>Pkd1</i> wild type and null MEK cells. The numbers at the bottom indicate the relative intensities of the bands, which are normalized to actin. (B and C) TNFα and RANKL stimulation decreased the expression of p21 mRNA (B) and protein (C). n = 3, ANOVA, p < 0.01. The numbers at the bottom indicate the relative intensities of the bands, which are normalized to actin. (D) Western blot analysis of the expression of Id2 and p21 from whole cell lysates of <i>Pkd1</i> wild type MEK cells treated with p38 activator U-46619 (5 μM). The numbers at the bottom indicate the relative intensities of the bands, which are normalized to actin. (E) <i>Pkd1</i> wild type MEK cells were transfected with control siRNA or Id2 siRNA for 24 hours, and then treated with TNFα and RANKL for 24 hours. The expression of Id2 and p21 in these cells were analyzed by western blot. The numbers at the bottom indicate the relative intensities of the bands, which are normalized to actin. (F) TNFα and RANKL stimulation increased the proliferation of <i>Pkd1</i> wild type MEK cells analyzed by MTT assay. n = 4, ANOVA, p < 0.01. (G) TNFα and RANKL mediated pathways in renal epithelial cells.