TNFAIP3 expression was repressed by Let-7 miRNAs in HEK293T cells.

<p>(A) HEK293T cells were transfected with two AGO2 specific siRNAs, 48 hours after transfection, cells were lysed and the expression of AGO2 and TNFAIP3 was detected by western blot. The band density was quantified by using Quantity One software and the results were analyzed by student’s t-test. *p<0.05, **p<0.01. (B) Luciferase reporter system was employed to screen the miRNAs may repress TNFAIP3 expression. Luciferase vector containing full length TNFAIP3 3’UTR and miRNA mimics were transfected into HEK293T cell. 48 hours after transfection, cells were lysed and luciferase activity was detected. *p<0.05, **p<0.01. (C) HEK293T cells were transfected with Let-7 miRNAs, 48 hours after transfection, cells were lysed and TNFAIP3 was detected by western blot. The band density was quantified by using Quantity One software and the results were analyzed by student’s t-test. *p<0.05, **p<0.01.