MED30 was overexpressed in gastric cancer tissues.

<p>(A-D) Immunohistochemical staining showed the overexpression of MED30 in gastric cancer tissues (B-D) versus normal gastric mucosae (A). Its overexpression was observed in invading cancer cells (C) and metastatic cancer cells nearby lymph nodes (D) in addition to the primary cancer site (B). (E) The overexpression of MED30 in gastric cancer cells was determined by real-time PCR using specific primers (NT; normal tissue). GAPDH was used to normalize all data. Values are the means ± SDs of the three independent experiments performed in triplicate. *, <i>p</i>< 0.01 (Student’s <i>t</i> test, versus NT). (F) The overexpression of MED30 in gastric cancer tissues was examined by real-time PCR using specific primers. GAPDH was used to normalize all data. Values are the means ± SDs of the three independent experiments performed in triplicate. *, <i>p</i>< 0.01; **, <i>p</i>< 0.05 (Student’s <i>t</i> test, versus normal gastric tissues). (G) MED30 protein expression in higher N stages (N stages 2 and 3) was significantly greater than in lower N stages (N stage 0, 1) (N = 41, <i>p</i>< 0.05, Mann-Whitney U test).