Regions surrounding cleavage sites for thrombin in a panel of important target molecules.

<p>Panel A shows the regions flanking the activating sites in natural substrates of thrombin. The amino acid sequences flanking both N-terminally and C-terminally of the cleavage sites for thrombin in FV, FVIII, PAR1,3 and 4, α & β fibrinogen and Protein C are depicted. Sequences are shown in a one-letter code and cleavage site numbering is based on the mature protein without signal peptide. The negatively charged amino acids are marked in red and thrombin cleavage sites are shown by arrows. Panels B-E shows schematic figures of FVIII, FV, fibrinogen and protein C showing the cleavage sites for thrombin (depicted by scissors with numbered residue).