GABA_A activation elicits similar response in GnRH neurons from NKCC1^-/- explants but is reduced by BUME.

<p>A) Example of a gramicidin perforated patch clamp recording used to measure response to MUS. Trace from NKCC1^-/- GnRH cell shows MUS depolarization is present before and after TTX. B) Peak amplitudes of MUS responses in GnRH neurons are similar in NKCC1^+/+ (+/+, black dots) and NKCC1^-/- (-/-, red dots) (B1) In contrast, BUME treatment significantly reduces the response to MUS in GnRH neurons in WT mice (B2). C) Reversal potential for GABA in GnRH neurons was evaluated by acute application of MUS (100 μM, arrowhead). All recordings were performed with CNQX/AP5 (10 μM each) and TTX (1 μM). Examples of recordings from GnRH neurons in untreated (WT) and bumetanide treated (+BUME) groups, and the voltage protocol used (C1). Reversal potentials were more negative when NKCC1 was inhibited by BUME (20 μM, red dots) than in control (WT, black dots) cells (*p<0.05, error bars = SEM) (C2).