Levels of HGS-interacting proteins and putative substrates in spinal cord extracts of 4-week-old <i>Hgs</i>^<i>+/+</i> and <i>Hgs</i>^<i>tn/tn</i> mice.

<p>(A) Representative immunoblot and (B) quantitation of the ESCRT-0 proteins HGS and STAM1, the ESCRT-I protein TSG101, the ESCRT-0 interacting proteins EPS15, and the receptor tyrosine kinases TrkB, TrkA and EGFR in the spinal cords of <i>Hgs</i>^<i>+/+</i> and <i>Hgs</i>^<i>tn/tn</i> mice. β-tubulin was used as a loading control. (C) qPCR analysis of <i>Hgs</i> and <i>Stam1</i> in the spinal cords of <i>Hgs</i>^<i>+/+</i> and <i>Hgs</i>^<i>tn/tn</i> mice. Levels are expressed relative to levels found in wild type <i>Hgs</i>^<i>+/+</i> mice. Symbols represent unpaired t-tests corrected for multiple comparisons using the Holm-Sidak method. Data are shown as mean ± SE. (D) Motor neuron counts from lumbar segments 4/5 from <i>Hgs</i>^<i>+/+</i> and <i>Hgs</i>^<i>tn/tn</i> mice. n = 3 mice per genotype. (E) Immunostaining of <i>Hgs</i>^<i>+/+</i> and <i>Hgs</i>^<i>tn/tn</i> L4/5 segments with GFAP. Scale bar, 100 μm. Data are shown as mean ± SE. n > 3 mice per genotype. ***p<0.001.