Viral DNA accumulation during the infection with HSV-1 KOS or <i>HSV-1</i> kinase mutants.

<p>(A) Vero cells were inoculated with wild type HSV-1 KOS or HSV-1 <i>ΔUS3</i>, <i>ΔUL13</i>, and <i>ΔUL13/ΔUS3</i> mutants at an MOI of 2.5 pfu per cell. At indicated time points, lysates were prepared and blotted onto a nylon membrane. The accumulation of viral DNA was assayed by hybridization to a ³²P-labeled <i>US6</i>-specific oligonucleotide probe. ACV-treated KOS served as a negative control. (B) Relative intensity of hybridization signals was quantified by phosphorimager analysis. Results are shown as fold change in hybridization signal intensity relative to the background ± standard deviation (SD) of two duplicate infections.