Most relevant differences in terms of gene expression in H9c2 undifferentiated myoblasts vs RA-differentiated H9c2 cells.

<p>H9c2 cells were differentiated for 5 days in a low serum concentrated medium daily supplemented with RA. Both groups, undifferentiated and RA-differentiated cells, were collected and analyzed as described in Material and Methods section. FC>2 corresponds to, at least, two-fold up-regulation of the gene, FC<2 corresponds to, at least, two-fold down-regulation of the gene in differentiated cells with RA. Data correspond to four separate experiments and differences are also represented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0129303#pone.0129303.g002" target="_blank">Fig 2</a>. Abbreviation: Dif–genes related with differentiation process to a cardiac phenotype; CellSig–genes encoding proteins related with cell signaling pathways; Metabol—genes related with metabolic activity; Cellcycle—genes related with proliferation activity; Calcium–genes related with cellular calcium handling.</p><p>Most relevant differences in terms of gene expression in H9c2 undifferentiated myoblasts vs RA-differentiated H9c2 cells.