Sorting of the combinatorial HN antigen yeast library with FACS.

<p>The induced yeast cells were sorted and analyzed with FACS. (A) The induced combinatorial HN antigen yeast library was stained with the chicken immunosera against NDV and analyzed with FACS before the first sorting. (B) The first-sorted combinatorial HN antigen yeast library was analyzed with FACS after being stained with the chicken immunosera against NDV. (C) The second-sorted combinatorial HN antigen yeast library was analyzed with FACS after being stained with the chicken immunosera against NDV. (D—I) 6 clones randomly selected from the second-sorted combinatorial HN antigen yeast library were induced with the galactose overnight at 30°C. The expression of HN antigens was evaluated with FACS after being stained with the chicken immunosera against NDV. The open and gray histograms refer to the negative control and experimental groups, respectively.