NKT-TCR-transfected γδT cells show cytotoxicity against glycolipid-expressing target cells and K562 cells.

<p>(A, B) To test the lysis of ligand-loaded target cells, the cytotoxic activity of γδT (A) or γδT-NKT-TCR(+) cells (B) against CD1d-HEK293, CD1d-HEK293/Gal, CD1d-HEK293/Zol was assessed by LDH assay at an E:T ratio of 10. (C, D) γδT (C) or γδT-NKT-TCR(+) cells (E) were stimulated by CD1d-HEK293/Gal, CD1d-HEK293/Zol or both and then assessed for cytotoxicity against K562. Data are mean ±SEM from triplicates and representative of 4 healthy donors with similar results. (**p<0.01, ***p<0.005)