Embryo survival after injection of algae.

<p>In order to evaluate the effect of algae in the embryo survival 3 different concentrations of <i>C</i>. <i>reinhardtii</i> (Low: 750 algae/ μl; medium: 2,500 algae/ μl and high: 10,000 algae/ μl) were injected into zebrafish embryos at 0 hpf (A) and 24 hpf (B). In both embryonic stages, results show a significant decrease in embryo survival with increasing concentrations of algae (C). In most cases a significant mortality was observed only the first days after injection. Error bar represents SEM. ** p ≤ 0.01; *** p≤ 0.001. n = 90 per group.