Detection of cell-surface B27₂ in leukocyte populations of <i>HLA-B27</i> transgenic rats at different ages.

<p><b>(A)</b> Representative flow cytometry analysis of cell-surface B27_<b>2</b> expression in leukocytes populations of Tg rats at different ages (6, 15, 23 and 30 weeks) from spleen and MLNs. WT leukocytes represent the control population where B27_<b>2</b> is absent. <b>(B)</b> MFI values plotted of positive B27_<b>2</b> stains from splenocytes. <b>(C)</b> MFI values plotted of positive B27_<b>2</b> stains from MLNs. Detection of cell-surface B27_<b>2</b> homodimers was performed using HD6-biotinylated and detected by streptavidin-APC. HD6 had been previously assessed as an antibody capable of recognizing cell-surface B27_<b>2</b> in human [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130811#pone.0130811.ref012" target="_blank">12</a>] and rat [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130811#pone.0130811.ref041" target="_blank">41</a>] leukocyte populations. Antibody panels: CD4+ T-cells (+CD3, +CD4), CD8+ T-cells (+CD3, +CD8), NK (+CD161a,—lineage), B cells (+CD45RA,-lineage) and Monocytes (+CD172a,—RP-1).