IH causes the accumulation of macrophages and upregulates β₃AR expression in the lungs.

<p>(A) Representative bright-field images of lung sections from the N and IH rats and images of immunofluorescent staining of such sections with anti-ED-1 antibody, anti-β_<b>3</b>AR antibody, or both (merged images). Calibration bar = 200 μm for 40 x, 50 μm for 200 x. (B, C) The numbers of ED-1- and β_<b>3</b>AR-positive cells per field (200 x) were counted using Image Pro Plus ver. 4.1 (n = 6 each, mean ± S.D.) (D) Ratio of the percentage of β_<b>3</b>AR-positive cells to the percentage of ED-1-positive cells (n = 6 each, mean ± S.D.) (E) Representative images of double immunocytochemical staining using anti-ED-1 and β_<b>3</b>AR antibody in BALF-derived macrophages. Calibration bar = 50 μm. (F) Western blot analysis of β_<b>3</b>AR in lung homogenate solutions from the N and IH rats (n = 6 each, mean ± S.D.) (G) The expression level of β_<b>3</b>AR mRNA in lung tissue samples from the N and IH rats (n = 6 each, mean ± S.E.M.) (H) Western blot analysis of β_<b>3</b>AR in BALF-derived pulmonary macrophages obtained after 6 weeks of IH or normoxic exposure (n = 5 each, mean ± S.D.) *Significant difference between the N and IH rats (*<i>P</i><0.05, **<i>P</i><0.01).