PDGFBB/FGF2-stimulated directional migration of Csf1r-EGFP+ phagocytes.

<p>Time-lapse, <i>in vivo</i> imaging of <i>Csf1r-EGFP</i>^<i>+/tg</i> corneas, starting at 3 hour post implantation, showed enhanced Csf1r-EGFP+ cell motility in response to PDGFBB/FGF2-releasing hydrogels as compared to blank or VEGF (a-c). From these movies (3 separate movies from 3 individual mice and each condition), Csf1r-EGFP+ cell density (d), total displacement (e) and migration velocity (f) were calculated and shown to be greater for the PDGFBB/FGF-implanted corneas. Time lapse imaging of directional migration of the Csf1r-EGFP+ phagocytes was performed at 3.5 hour post implantation (g) and analyzed by Imaris (h). The displacement and angle of displacement of each Csf1r-EGFP+ cell was plotted on a rose plot (i) and indicated that the Csf1r-EGFP+ cells stimulated by PDGFBB/FGF2 migrated toward implanted hydrogels.