Assessment of heterogeneous molecular weight shift of proteins revealed by deep purple (DP) staining.

<p>The proteomic map has been labeled by Cy3 (green) and stained DP (red) (A). This process revealed a predominant shift of low molecular weight proteins, with the Cy3-labeled proteins appearing heavier than the DP-stained proteins (B). The shift seemed weaker and could even disappear in the case of higher molecular weight proteins (C) but this observation did not apply to all high MW proteins (D). Moreover, some proteins were stained only by Cy3 and others only by DP (arrows in E).