Cell viability and cell recovery in fresh and frozen–thawed cells.

<p>(A) Cell viability of fresh and frozen-thawed cells as determined by trypan blue dye exclusion immediately after thawing. (B) Percentage of cell recovery of frozen–thawed cells after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72 h (hPBMCs and mBMCs) of culture. (C) Cell viability as determined by trypan blue dye exclusion after 24 h (MEF, C18-4, CHO, HEK, and mES cells) and 72h (hPBMCs and mBMCs) of culture. Data represent mean ± SEM off our trials for each cell type in each group. Bars with different letters are significantly different at P < 0.05.