Surface plasmon resonance analysis of HpDprA, HpRF and HpDML1 interaction with DNA.

<p>Different concentrations of full length HpDprA <b>(A)</b>HpRF<b>(C)</b> and HpDML1<b>(E)</b> were injected on to the immobilized single stranded DNA (90 mer, ~1000 RU) surface in buffer containing 150 mM NaCl. Both sample injection and buffer injection were carried out as described in materials and methods. Similarly, binding sensorgrams were obtained for the interaction of dsDNA (90 bp, ~1000 RU) with full length HpDprA <b>(B)</b>HpRF<b>(D)</b> and HpDML1<b>(F)</b>. Sensorgrams depicting changes in response unit (the y—axis) as a function of time (the x—axis) are shown. The concentrations of soluble analytes and affinity constant (K_d) values are indicated in the inset to the figures.