SDS-PAGE analysis of secreted RKOD in the cell culture supernatants of the shake flasks and soluble KOD and KOD-S expressed in <i>E</i>. <i>coli</i>.

<p>(A). M, protein molecular weight marker (the size of each band is indicated on the left); lane 1, cell culture supernatant (15 μl) of the strain bearing the pHBM905A plasmid (negative control); lanes 2–7, cell culture supernatants (15 μl) collected from days 1–6, respectively; and lane 8, purified RKOD; (B). M, protein molecular weight marker (the size of each band is indicated on the left); lane 1, purified KOD; lane 2, purified KOD-S.