The appearance of bipolar spindles is delayed in S574A and DEL cells following MT regrowth.

<p>(A) MT regrowth assays in DT40 cells with genotypes as indicated. MTs were depolymerized by nocodazole and then allowed to recover for 3 or 15 minutes. Note that the mutant TACC3 products accumulate on nascent MTs. Scale bar = 5 μm. (B) Graph depicts percentages of mitotic cells with discernible bipolar spindles after 15 minutes of MT regrowth. n = 3 independent experiments; minimum of 100 cells scored per genotype per experiment. Student t-test (* P = 0.03).