Effect of EDCs alone and in combination on the secretion of TNF-α by differentiated THP-1 cells.

<p>Differentiated THP-1 cells were exposed to BPA, DEHP, DBP 4-OP or a combination of both for 24 hours. Culture medium was replaced by fresh medium containing 10 ng/ml of LPS for a subsequent 24 hours of incubation. TNF-α concentrations were determined using a sandwich ELISA test. Values are means ± SD (N = 3). Each experiment has been performed three times and in triplicate. a: <i>vs</i> control (Tukey’s test, p<0.05); b: <i>vs</i> combination (One-way ANOVA, Tukey post test, p<0.05).