SPI and DCP1 interact at P-bodies in BiFC assays.

<p>Fusion proteins are expressed under the control of the 35S promoter. (A) Interaction of YFP_N-SPI and YFP_C-DCP1 (left) in transiently transformed <i>N</i>. <i>benthamiana</i> leaves 72 h post-transfection at DCP2-mCHERRY labeled P-bodies (middle). Right pictures show the overlay of the left (green) and middle (magenta) pictures. (B) Interaction of YFP_N-SPI and YFP_C-DCP1 in cytoplasmic dots (left) in <i>N</i>. <i>benthamiana</i> leaves coexpressing free RFP as transformation control (middle). Right pictures show the corresponding transmission picture. (C–F) Representative images of BiFC negative controls. No YFP signal in leaf epidermis cells coexpressing YFP_N-SPI-PBW and YFP_C-AtMYC1 in combination with DCP2-mCHERRY (C) and free RFP (D). No YFP signal in leaf epidermis cells coexpressing YFP_C-DCP1 and VPS25-YFP_N in combination with DCP2-mCHERRY (E) and free RFP (F). Scale bar: 50 μm.