Morphological characteristics of cells lacking myosin II isoforms Collagen constructs containing cells were prepared and imaged as outlined.

<p>IMARIS image analysis software was used to quantify the observations made on cell morphology in 3D collagen gels across three separate experiments. <b>(A)</b> The average number of protrusions per cell were measured using the Filaments function in IMARIS. Statistical significance was calculated using one-way ANOVA with a Tukey post-test. The difference between parental and IIA KD cell types was significant (p < 0.01), as was the difference between IIA and IIB KD cells (p < 0.001). <b>(B)</b> The sphericity of the cell bodies was calculated using the Surfaces function in IMARIS. IIA KD cells were slightly more spherical than parental or IIB KD cells, though the difference was not statistically significant. <b>(C)</b> The elongation factor of the cell bodies was calculated using the measurements function in IMARIS. We defined the elongation factor as the measurement of the longest dimension of the cell body, divided by the measurement of the shortest dimension. IIB KD cells had a slightly higher elongation factor than the parental or IIA KD cells, though the difference was not statistically significant.