Serial transmission of CJD-102L prions in transgenic mice homozygous for 102L or wild type human PrP.

<p>IHC = immunohistochemistry; IB = immunoblotting; ND = not determined; Tg35c and Tg152c are congenic on FVB/N genetic background; sCJD = sporadic CJD; iCJD = iatrogenic CJD; DM, = dura mater; GH = growth hormone.</p><p>* All inocula were first passaged in 102LL Tg27 transgenic mice to generate CJD-102L prions.</p><p>^† All samples were analysed by both IB and IHC using monoclonal antibodies ICSM 18 and ICSM 35. In 102LL Tg27 tissues ICSM 35 serves as a control to define non-specific background.</p><p>^# Positive either by clinical signs, immunoblotting and/or immunohistochemistry.</p><p>^‡ All samples were positive for ICSM 18 but negative for ICSM 35.</p><p>^§ Only 1 sample was available for analysis by IB.</p><p>Serial transmission of CJD-102L prions in transgenic mice homozygous for 102L or wild type human PrP.