Downregulation of PODXL and CLIC5 affects migration and invasion in Huh7 cell lines.

<p>A) (Left) Expression of PODXL and CLIC5 was examined by Western blot analysis in transduced Huh7 cells. GADPH was included as a loading control. (Right) Proliferation of control and transfected Huh7 cells was analyzed using an MTT assay. B) (Up) Representative images of the wound assay in Huh7, shRNAPODXL and shRNACLIC5 cells at 0, 24, 48 and 72 h (20x magnification) (Down) Quantification of gap closure. The graph represents the wound width as the mean in % of the closure of original wound at 0, 24, 48 and 72h. Similar results were obtained in quadruplicate plates. C) The migration capacities of Huh7, shRNAPODXL and shRNACLIC5 cells were measured using the cell migration assay. The migrated cells were fixed, stained with crystal violet, and measured using colorimetry. D) The invasion capacities of Huh7, shRNAPODXL and shRNACLIC5 cells were measured using the ECMatrix Cell Invasion assay. The invasive cells were fixed, stained with crystal violet, and measured using colorimetry. Error bars indicate the standard error of the mean (SEM). *p≤0.05 vs. untreated control values (Tukey–Kramer test). Each experiment was repeated at least four times.