NAC attenuates H₂O₂-induced caspase activation, but shows limited effect on doxorubicin-induced caspase activation.

<p><b>(</b>A) Representative image (top) and quantitative analysis (bottom) of Western blot of cleaved caspase 3 in cell lysates from attached WT MEFs treated with increasing concentrations of H_<b>2</b>O_<b>2</b> and/or 2 mM NAC for 4 h. Equal amount of proteins was loaded. (B) Representative image (top) and quantitative analysis (bottom) of Western blot of cleaved caspase 3 in cell lysates from attached WT MEFs treated with 3 μM doxorubicin and/or 2 mM NAC for 8 or 16 h. n = 4–6 for each condition. <b>*</b><i>P</i> < 0.05 vs. control. ^#<i>P</i> < 0.05 vs. H_<b>2</b>O_<b>2</b> or doxorubicin only condition.