Nifedipine and diazoxide reduced cell apoptosis in PA-impaired islets.

<p>(A) Cultured islets were treated with PA in the presence/absence of different compounds for 48 h, then TUNEL staining was performed. Red fluorescence nuclei indicate apoptotic cells. Blue fluorescence showed all nuclei. Scale bar = 100 μm and referred to all panels. (B) Apoptotic rate was calculated as TUNEL-positive cell number modified by islet area. 10 islets were analyzed from six duplicated wells. * p<0.05; ** p<0.01 denote significant difference versus the PA-treated alone group, n = 6.