β-actin promoter-driven SRG3 over-expression increases both the basophil and mast cell numbers and IL4 production.

<p>(A) Splenocytes were isolated from the spleens of WT, CD2-SRG3 Tg, and β-actin-SRG3 Tg B6 mice at either 8 or 20 weeks of age. The frequencies of both mast cells (FcεRI⁺CD200R^-CD3^-B220^-) and basophils (FcεRI⁺CD200R⁺CD3^-B220^-) in the spleen were plotted (upper left panel). The absolute cell numbers of both basophils and mast cells were determined (upper right panels). The means ± SD are shown (n = 3–4; **P<0.01, ***P<0.001). (B) Splenocytes from WT, CD2-SRG3 Tg, and β-actin-SRG3 Tg B6 mice at the age of either 8 or 20 weeks were cultured in the presence of recombinant mIL3 (20 ng/ml) for 24 hrs. The percentages of IL4-producing cells among both basophils and mast cells were analyzed via flow cytometry. The mean values ± SD are shown (n = 3–4; *P<0.05, **P<0.01). (c) Splenocytes were isolated from the spleens of WT, CD2-SRG3 Tg, and β-actin-SRG3 Tg B6 mice at either 8 or 20 weeks of age. The absolute cell numbers of both IL4⁺ basophils and IL4⁺ mast cells were determined. The means ± SD are shown (n = 3–4; **P<0.01, ***P<0.001).