Infiltration of Treg cells into the spinal cord was dramatically increased in β-actin-SRG3 Tg mice compared to CD2-SRG3 Tg mice during EAE pathogenesis.

<p>(A) Splenocytes and (B) MNCs were prepared from the spinal cord of both MBP TCR Tg B10.PL mice and CD2-SRG3/MBP TCR double Tg B10.PL mice immunized with MBP to induce EAE. The percentages of CD25⁺FoxP3⁺ Treg cells among the total CD4⁺ T cell population in splenocytes and MNCs were evaluated via flow cytometry (upper panel). Alternatively, splenocytes and MNCs were prepared from the spinal cord of both MBP TCR Tg B10.PL mice and β-actin-SRG3/MBP TCR double Tg B10.PL mice immunized with MBP to induce EAE. The percentages of CD25⁺FoxP3⁺ Treg cells among the total CD4⁺ T cell population in splenocytes and MNCs were evaluated via flow cytometry (lower panel). The mean values ± SD are shown (n = 5; *P<0.05).