Immunofluorescence staining of the mammary cell markers in MCF10A cells in 2D culture.
- Ying Qu (437588)
- Bingchen Han (765679)
- Yi Yu (28902)
- Weiwu Yao (726029)
- Shikha Bose (765680)
- Beth Y. Karlan (184236)
- Armando E. Giuliano (765681)
- Xiaojiang Cui (580571)
Publication date
July 2015
DOIAbstract
<p>MCF10A cells grown in monolayer were fixed and subjected to immunofluorescence staining using the antibodies against basal cell <b>(A)</b>, luminal cell <b>(B)</b>, and breast tissue markers <b>(C)</b>. Secondary antibodies were either AF488 (green)—or AF594 (red)-conjugated. DAPI was used for nuclear staining. Bars: 50μm. Original magnification: ×200.</p
Add to ORKG