<i>Alkbh8</i>^<i>-/-</i> cells have elevated reactive oxygen species (ROS).

<p>A) Intracellular ROS was measured in immortalized MEFs by DCFDA staining. Representative images are shown for cell batches taken from the peak frequency of DCFDA intensities for wt and <i>Alkbh8</i>^<i>-/-</i> MEFs. B) The frequency of wt (blue) and <i>Alkbh8</i>^<i>-/-</i> (green) cells exhibiting specific DCFDA intensities is plotted for a population of 6,000 cells. C) Intracellular ROS was measured in immortalized MEFs by DCFDA staining 6 h after the indicated treatments and is shown as median fluorescence intensity emitted by oxidized DCFDA at 517–527 nm +STDV, n = 3).