Administration of A2BR antagonist to IFN-γ^-/- mice mitigated the severity of induced EAU and the activation of Th17 autoreactive T cells.

<p>(A&B). IFN-γ^-/- mice were immunized with IRBP1-20/CFA alone or were also injected with an A2BR antagonist (MRS 1754, 2 mg/kg) via i.p., on days 1, 4, 7, and 10 post-immunization. The control mice received an injection of vehicle (diluted DMSO). EAU was scored by fundoscopy. All the mice were euthanized 30 days post-immunization, when the mice were sacrificed and the eye were taken and subjected to pathological examination. C) Surface staining assessing abundancy of γδ T cells among CD3⁺ splenic cells and CD44⁺ and CD44^- γδ T cells. D). Cytoplasmic staining assessing the percentage of IL-17⁺ cells among the in vitro antigen-stimulated IRBP-specific T cells, detailed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132348#pone.0132348.g001" target="_blank">Fig 1</a> legend. E). ELISA results from 48-hour culture supernatants were assessed for IL-17. Data are from one single experiment, which are representative of three independent experiments. **p< 0.01.