HBGA-binding of wild type and various mutant P particles with single amino acid mutations at the HBGA-binding interface of OIF virus.

<p>(A), Binding of wild type OIF P particles with a panel of oligosaccharides representing different HBGAs (A, B, Le^a, Le^b, Le^x, Le^y, H1, H2, H3, sialyl Le^a and sialyl Le^x) and the type 1 (Pre1) and type 2 (Pre 2) precursors. (B to L), Binding of various mutant P particles with single amino acid mutations at the HBGA-binding interface with the same panel of oligosaccharides as in (A). The concentrations of the P particles were 10 μg/ml, while oligosaccharides for plate coating at 2 μg/ml. Y axes indicate the binding signals in optical densities at 450 nm (OD450), while the X-axes indicate different oligosaccharides.