DPY30 regulated the proliferation of gastric cancer cells.

<p>(A) Real-time PCR was used to determine the efficiency of knockdown or overexpression of DPY30 in HFE145, SNU1, SNU16, SNU216 and SNU638 cells. Knockdown efficiency was determined after transfecting cells with 100 nM DPY30 siRNA targeting the ORF or scrambled siRNA (SCR). Overexpression efficiencies were determined in DPY30-overexpressing and mock cells. (B) Effect of DPY30 knockdown or overexpression on cell proliferation. A cell viability assay was used to measure cell proliferation in the presence of 1% FBS. For the DPY30 knockdown experiments, cell viability assays were performed five days after transfecting 100 nM DPY30 siRNA or SCR. After three days of culture, cell viability assays were performed on DPY30-over and mock cells. (C) Expression analysis of DPY30 after known-down or overexpression by siRNAs or DPY30 ORF respectively. The ORF-targeting or the 3’-UTR-targeting siRNA was used for the knock-down. (D) Exogenous DPY30-ORF rescued the inhibition of proliferation by the 3’-UTR-targeting DPY30 siRNA. Mock or DPY30-overexpressing cells were transduced with two kinds of DPY30 siRNA (3’-UTR-targeting or ORF-targeting), then cell viability assay was examined. Values are the means ± SDs of three independent experiments performed in triplicate. *, <i>p</i> < 0.01 (Student’s <i>t</i> test, versus SCR or Mock).