Th2 cells become IL-12 responsive following adoptive transfer.

<p>A and B). CD4⁺<i>Il4</i>^<i>gfp+</i><i>in vitro</i> Th2 cells or naïve CD4 cells were transferred to <i>Rag1</i>^{<i>–/–</i>}mice for 2 weeks. CD4⁺TCRβ⁺ cells were then sorted from spleens of recipient mice and treated with 10ng/mL IL-12 for 15 minutes (blue) (or untreated, red) and then stained for pSTAT4 by FACS. Representative of 2 independent experiments. C—G). Naïve CD4⁺ T cells or <i>in vitro</i> Th2 cells (CD4⁺TCRβ⁺<i>Il4</i>^<i>gfp+</i><i>Ifng</i>^<i>yfp-</i><i>Il17a</i>^{<i>FP635-</i>}) were transferred to <i>Rag1</i>^{<i>–/–</i>}recipient mice for 14 days. Mice were infected with <i>P</i>. <i>chabaudi</i> and harvested at day 8 post-infection. Mice were treated i.p. with 0.5mg of anti-IL12 at days -1, 6, 13, and 19. D–F). Cytokine reporter expression in transferred cells in the spleen, with or without anti-IL-12 treatment. G). Percent parasitemia, determined by blinded counting of Giemsa-stained blood smears. Data are representative of 2 independent experiments with 3–6 mice per group. * denotes P<0.05.