Converted Th2 cells are transcriptionally similar to Th1 cells.

<p>Purified <i>in vitro</i> Th2 cells (CD4⁺TCRβ⁺<i>Il4</i>^<i>gfp+</i><i>Ifng</i>^<i>yfp-</i><i>Il17a</i>^{<i>FP635-</i>}) or naive CD4⁺ T cells were sorted for RNA or transferred to <i>Rag1</i>^{<i>–/–</i>}mice. Recipients were then infected with 10⁵<i>P</i>. <i>chabaudi</i>, as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004994#ppat.1004994.g002" target="_blank">Fig 2</a>. CD4⁺TCRβ⁺<i>Ifng</i>^<i>yfp+</i><i>Il4</i>^<i>gfp-</i><i>Il17a</i>^{<i>FP635–</i>} cells were then sorted from spleens of recipient mice at day 8 post-infection for RNA. RNA sequencing and IPA analysis was performed on the four cell populations. Data were expressed relative to naïve in A and B. A and B). Venn diagram and heatmap generated from differentially regulated genes (P<0.05, 2-fold relative to naïve T cells) of Th2 cells and Th1 cells, highlighting 1899 genes commonly expressed, which were not changed in Th2 cells. Th2 cells and converted Th2 cells shared 117 differentially regulated genes, which were not expressed in Th1 cells. C, D, and F). Normalized RNA-Seq reads of indicated genes. E). Upstream pathways analysis in Ingenuity Pathways Analysis (IPA) identified IL-12, type 1 IFN, and IFNγ as potential upstream regulators of converted Th2 cells. Samples were generated from 3 biological replicates (each sample representing cells from a single donor mouse).