Ion channel events and their current voltage relationship.

<p>(a) Representative single channel current traces for the Na_vSp pore at different applied transmembrane potentials. (b) I-V plot for the Na_vSp pore. Error bars are one standard deviation for ≥ 3 individual bilayer measurements. The electrophysiology buffer is 0.5 M NaCl, 10 mM HEPES, pH 7.4. Channels were introduced in the aperture-suspended bilayer of POPE:POPG (1:1) by fusion of proteoliposomes with a lipid-to-protein ratio of 300:1. Data were recorded at a sampling frequency of 1.25 MHz and were low pass-filtered at 1.25 kHz.