Lung injury features.

<p>There was no interaction between GLN pretreatment and IL-1/LPS insufflation on BAL protein concentrations (1a). IL-1/LPS significantly increased BAL protein levels in both GLN+ and GLN- groups. GLN+IL-1/LPS+ groups had significantly lower BAL protein concentrations than GLN-IL-1/LPS+ groups. GLN and IL-1/LPS showed a significant interaction on BAL LDH concentration (1b). BAL LDH concentrations were significantly higher in GLN-IL-1/LPS+ groups compared to both IL-1/LPS- groups. GLN+IL-1/LPS+ rats had significantly lower BAL LDH levels than GLN-IL-1/LPS+ groups. Fig 1c shows representative H&E histology images from each group. IL-1/LPS insufflation increased the blindly assessed histological lung injury scores with no interaction or effect by GLN pretreatment (1d). (The significance of differences among the four groups was analyzed by two-way ANOVA followed by paired comparisons. Statistical significance was accepted as p<0.05: * compared to GLN-IL-1/LPS- group, # compared to respective GLN+IL-1/LPS- group, ^ compared to GLN-IL-1/LPS+ group).