Microbiome composition and biodiversity are altered by genotype and diet.

<p>Fecal microbiomes from WT and Plin2-null mice on standard chow, low fat (LF), and high fat (HF) diets were profiled by high-throughput 16S rRNA amplicons sequencing. Panel A displays the distributions of bacteria among phyla (left) and family-level taxonomic categories (right). For simplicity, only taxa with mean relative abundances >2% are displayed; numerical values are the median within-group abundances of taxa. Overall p-values (P_overall) were ascertained by non-parametric PERMANOVA tests for all six diet/genotype groups, using Morisita-Horn dissimilarity scores. PERMANOVA using both diet and genotype as predictor variables was performed to calculate adjusted p-values (P_diet and P_genotype). Significant results of pairwise PERMANOVA tests performed on each pair of genotype/diet groups are indicated by horizontal bars above each heatmap: •: p<0.1; *: p<0.05; **: p<0.01; ***: p<0.001. Panel B displays the differences in biodiversity indices (richness, evenness, and complexity) by genotype and diet categories. Overall p-values (P_overall) were ascertained by ANOVA tests for all six diet/genotype groups. ANOVA using both diet and genotype as predictor variables was performed to calculate adjusted p-values (P_diet and P_genotype).