SR and DR selection increases body size and alters metabolic profile.

<p>A) Schematic of selection processes for SR and DR flies. Adult outbred flies were placed under desiccation or starvation conditions until ~15% of the flies survived. The flies were then moved to food. This process was repeated over >80 generations. The F_DR controls were placed on agar during desiccation selection to account for food deprivation in DR selected flies. There were three replicated SR populations (designated SR_a, SR_b and SR_c) and three fed control populations (F_SRa, F_SRb and F_SRc). For DR experiments there were three replicated groups (designated DR_a, DR_b and DR_c) and three fed control populations (F_DRa, F_DRb and F_DRc). B) Flies from the C Group. SR_c and DR_c flies are visibly larger than F_SRc and F_DRc controls. C) Triglyceride levels are elevated in the SR_c flies compared to F_SRc controls. No differences are observed between DR_c flies and F_DRc controls (P<0.001; See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131275#pone.0131275.s004" target="_blank">S1 Table</a>). D) Glycogen levels were greater in DR_c flies than in F_DRc controls. No differences were present between SR_c flies and F_SRc controls (P<0.001; See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131275#pone.0131275.s004" target="_blank">S1 Table</a>). E) Free glucose levels did not differ between SR_c or DR_c flies and their respective controls (P>0.05; See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131275#pone.0131275.s004" target="_blank">S1 Table</a>).