Effect of estradiol on CNP-induced meiotic arrest and the expression of Npr2.

<p>A, Effect of estradiol on CNP-induced meiotic arrest. COCs were cultured in medium containing 100 ng/mL CNP and different concentrations (0, 5, 10 and 50 nM) of estradiol. The proportion of oocytes with GV was evaluated after 6 and 8 hours of culture, respectively. B, Effect of estradiol on the expression of Npr2 mRNA. The Npr2 mRNA in COCs cultured in medium containing 100 ng/mL CNP and 10 nM estradiol was detected after 0, 2, 4, 6 and 8 hours of culture, respectively. CNP: C-type natriuretic peptide; Npr2: natriuretic peptide receptor 2; COCs: cumulus oocyte complexes; E2: estradiol.