Adjuvant nanoparticle anti-IL6 siRNA suppresses hepatic ablation-induced distant subcutaneous R3230 tumor growth via reduction in VEGF-mediated angiogenesis.

<p><b>(A)</b> Tissue levels of VEGF (Y-axis², dark gray columns) in the periablational tissue surrounding the ablation zone were quantified at 72hr after different treatments (mean ± standard deviation, n = 3–4 animals/arm). Hepatic thermal ablation increased periablational VEGF levels, which were then suppressed with adjuvant MNP anti-IL6 siRNA (p<0.05 for all comparisons). <b>(B-E)</b> Similarly, hepatic thermal ablation led to increased microvascular density/angiogenesis (immunohistochemistry for CD34, A: Y-axis¹, light gray columns) in distant subcutaneous R3230 tumor that was also suppressed with adjuvant anti-IL6 siRNA (n = 6–7 animals/arm).