Reactivity of scFv-LT recombinant antibody against LT toxin.

<p>(A) Nitrocellulose membrane containing the LT toxin protein after SDS-PAGE 15% were subjected to immunoblotting with antibodies. Lanes: <b>1.</b> scFv-LT 200 μg/mL, <b>2.</b> 50 μg/mL anti-LT mAb. (B) Immunofluorescence assay after LT interaction with Y-1 cells. Panel 1. Reactivity with scFv-LT; Panel 2. Reactivity with anti-LT mAb. Panel 3. Reactivity with scFv-LT in the absence of LT toxin. Panel 4. Light microscopy. (C) scFv-LT titration with LT toxin by capture ELISA. (D) Detection of LT in culture supernatants of LT- and LT/ST-producing ETEC isolates by capture ELISA.