<p>A) Large scale purification and identification of recombinant CCR5-T4L. Recombinant CCR5-T4L was expressed in <i>E</i>. <i>coli</i>. The pET-20b expression vector was transformed into Rosetta 2 (DE3) golden BL21 pLysS cells and analyzed using Coomassie brilliant blue R-250. Lane M: protein marker; lane 1: uninduced bacterial lysate; lane 2: IPTG-induced bacteria lysate; lane 3: small amount of soluble fraction purified on a Ni-nitrilotriacetic acid (NTA) histidine-binding column; lane 4: small amount of membrane fraction purified using a Ni-nitrilotriacetic acid (NTA) histidine-binding column; lane 5: large amount of soluble fraction purified by fast protein liquid chromatography (FPLC) using an AKTA purifier; lane 6: large amount of mem...
Escherichia coli is a favored host for rapid, scalable expression of recombinant proteins for academ...
<p>Bacterial α(2,6)-ST genes (Pd-ST, P224-ST, and P145-ST) were cloned into a pCold II vector for re...
High-level expression of a soluble form of pencillin-binding protein 5 (PBP5), called PBP5s, and tra...
<p>Lane M: protein marker; Lane 1: total soluble cell lysate; Lane 2: flow-through elution; Lane 3: ...
Chemokine receptors belong to a class of integral membrane G-protein coupled receptors (GPCRs) and a...
<p>Expression and purification analysis of recombinant proteins Lsa46 <b>(A)</b> and Lsa77 <b>(E)</b...
<p>Lane 1: uninduced BL21 bacteria with pET-30a (+) vector, Lane 2: induced BL21 bacteria with pET-3...
Recombinant BOR protein expressed in the E. coli BL21 (DE3) Omp8 Rosetta strain at various pH values...
<p>A) ChrB was overexpressed with a carboxy-terminal 6-histidine tag in <i>E. coli</i> BL21 (DE3). L...
Protein expression and purification have traditionally been time-consuming, case-specific endeavors,...
Crude recombinant BOR was separated by 12 per cent SDS-PAGE and stained with Coomassie brilliant blu...
Chemokine receptors belong to a class of integral membrane G-protein coupled receptors (GPCRs) and a...
<p>(A) Overexpression of <i>w</i>Bm-LigA in Rosetta <i>E. coli</i> cells<b>.</b> lane M, standard pr...
<p>Coomassie blue stained 12% SDS-PAGE. Lane 1: uninduced lysate; Lane 2: IPTG induced lysate; Lane ...
RecBCD was purified from E. coli JM109 co-transformed with plasmids pPB800 and pPB520, which were a ...
Escherichia coli is a favored host for rapid, scalable expression of recombinant proteins for academ...
<p>Bacterial α(2,6)-ST genes (Pd-ST, P224-ST, and P145-ST) were cloned into a pCold II vector for re...
High-level expression of a soluble form of pencillin-binding protein 5 (PBP5), called PBP5s, and tra...
<p>Lane M: protein marker; Lane 1: total soluble cell lysate; Lane 2: flow-through elution; Lane 3: ...
Chemokine receptors belong to a class of integral membrane G-protein coupled receptors (GPCRs) and a...
<p>Expression and purification analysis of recombinant proteins Lsa46 <b>(A)</b> and Lsa77 <b>(E)</b...
<p>Lane 1: uninduced BL21 bacteria with pET-30a (+) vector, Lane 2: induced BL21 bacteria with pET-3...
Recombinant BOR protein expressed in the E. coli BL21 (DE3) Omp8 Rosetta strain at various pH values...
<p>A) ChrB was overexpressed with a carboxy-terminal 6-histidine tag in <i>E. coli</i> BL21 (DE3). L...
Protein expression and purification have traditionally been time-consuming, case-specific endeavors,...
Crude recombinant BOR was separated by 12 per cent SDS-PAGE and stained with Coomassie brilliant blu...
Chemokine receptors belong to a class of integral membrane G-protein coupled receptors (GPCRs) and a...
<p>(A) Overexpression of <i>w</i>Bm-LigA in Rosetta <i>E. coli</i> cells<b>.</b> lane M, standard pr...
<p>Coomassie blue stained 12% SDS-PAGE. Lane 1: uninduced lysate; Lane 2: IPTG induced lysate; Lane ...
RecBCD was purified from E. coli JM109 co-transformed with plasmids pPB800 and pPB520, which were a ...
Escherichia coli is a favored host for rapid, scalable expression of recombinant proteins for academ...
<p>Bacterial α(2,6)-ST genes (Pd-ST, P224-ST, and P145-ST) were cloned into a pCold II vector for re...
High-level expression of a soluble form of pencillin-binding protein 5 (PBP5), called PBP5s, and tra...