Geraniol action on viability of HDMEC.

<p>A, B: Cell viability (% of control) (A) and cytotoxicity (% of control– 100%) (B) of HDMEC, which were exposed for 24h to different doses (50–400μM; n = 4) of geraniol, Triton X-100 as cytotoxic control (TX) or vehicle (control; n = 4), as assessed by WST-1 assay (A) and LDH release assay (B). Means ± SEM. *P<0.05 vs. control. C-E: Representative graphs from flow cytometry analyses of PI- and annexin V-stained HDMEC, which were exposed for 24h to 200μM (D; n = 4) and 400μM (E; n = 4) geraniol or vehicle (control; C; n = 4). F-H: Viable cells (= PI-negative/annexin V-negative; %) (F), necrotic cells (= PI-positive/annexin V-negative; %) and apoptotic cells (PI-negative/annexin V-positive and PI-positive/annexin V-positive; %), as assessed by flow cytometry. Means ± SEM.