CM gene expression dynamics in the fat body of adult female mosquitoes.

<p>(A) qPCR analysis of selected CM genes during PE and PBM. Relative abundance of PE and PBM time points were normalized to the 0-6h PE and 72h PE, respectively. In the graphs, the abundance of these two time points is represented as 1.0, with corresponding adjustments for other time points. All experiments were performed in triplicate, with similar results. Error bars represent ± SD. * <i>p</i> < 0.05;** <i>p</i> < 0.01. Additional CM genes are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005309#pgen.1005309.s002" target="_blank">S2 Fig</a> (B) Western blot showing the fat body protein levels of key CM enzymes during PE and PBM. Total protein extracts were prepared from eight adult female fat bodies for each indicated time point. Actin was used as a control for loading and transfer.