Changes in the level of intermediary metabolites throughout the reproductive cycle of adult female mosquito.

<p>(A and B) Relative levels for intermediary metabolites of glycolysis (A) and the citrate cycle (B) during PE and PBM development. Glucose-6-phosphate, fructose-6-phosphate, pyruvate and lactate from the glycolytic pathway, and citrate, succinate fumarate and malate from the citrate cycle were measured using gas chromatography—mass spectrometry (GC/MS). The time points 0-6h PE and 72h PE were used as controls for PE and PBM periods, respectively. The controls are represented as relative abundance 100, and the levels at other time points were adjusted accordingly. In the box plots, the box represents the lower and upper quartiles, the horizontal line represents the median and the bars represent the minimum and maximum data points (n = 12 samples collected from independent populations, with six adult female mosquito per sample). Three independent sets of metabolite measurements were performed with similar results.