Antagonist activity in mice.

<p>Antagonist activity in mice was determined by their ability to induce or inhibit IFN-induced gene production 3 hours after injection. (A) WT C57bl/6 mice were injected with 1 μg of human (Hu) or mouse (m) IFNβ or 50 μg of each of the antagonists (1ant stands for IFN-1ant and the other antagonists are on top of the 1ant mutations). qPCR of the liver cDNA was performed to evaluate the gene induction levels of three robust (MX1, OAS2 and IFIT1) and two tunable (CXCL10 and CXCL11) genes. (B) WT C57bl/6 mice were injected I.P. with mIFNβ alone or in combination together with 50 μg of each of the antagonist. (C) HyBNAR mice were injected with 50 μg IFN-1ant, 1 μg HuIFNβ or a combination of the two. (D) Statistical analysis of the results in panel B (for genes that are differentially regulated by IFNβ versus IFN-1ant—marked in red) using Anova and Tukey post hoc test. Bars show mean difference between the treatments and error bars show 0.05 confidence intervals between the different treatments. The confidence interval for IFNβ vs combination is 0.005.