Antiviral activity of IFN antagonists in WISH and OVCAR3 cells.

<p>(A) WISH cells were treated with the antagonists, YNS, IFNα2 or left untreated for four hours. VSV virus was added for 18 hours, which after cell survival was monitored by crystal violet staining. (B) OVCAR3 cells were treated with the antagonists, YNS, IFNα2 or left untreated for four hours. EMCV virus was added for 23 hours, which after cell survival was monitored by crystal violet staining. The experiments were performed 3 times and the fitting results (for the amplitude) are reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130797#pone.0130797.t001" target="_blank">Table 1</a>. (C) Reduction in antiviral activity upon combined treatment of WISH cells with 200 nM antagonists combined with 0.0001–100 pM IFNα2.