Lymphocyte and macrophage infiltration into the liver of different mouse groups after 14 weeks of dietary intervention.

<p>SD group: control mice receiving a standard diet (SD) plus placebo; HFD group: obese mice receiving a high-fat diet (HFD) plus placebo; SD+Bif group: control mice receiving SD and a daily dose of 1 x 10⁹ CFU <i>B</i>. <i>pseudocatenulatum</i> CECT 7765; HFD+Bif group: obese mice receiving HFD and a daily dose of 1 x 10⁹ CFU <i>B</i>. <i>pseudocatenulatum</i> CECT 7765 by gavage during 14 weeks (n = 10). The ratio of CD8+/CD4+ of total CD3+ (<i>a</i>), T regulatory (CD4+CD25+Foxp3+) (<i>b</i>), T cytotoxic (CD3+CD4-CD8+) (<i>c</i>) and B lymphocytes (CD19+) (<i>d</i>) as well as total of macrophages (F4/80+) (<i>e</i>) and their subtypes M1 (F4/80+CD11c+CD206-) and M2 (F4/80+CD11c-CD206+) (<i>f</i>) were analyzed in each mouse group (n = 10 per group) by flow cytometry. Data are expressed as means and standard error (error bars) and statistically significant differences were determined by ANOVA with Bonferroni’s <i>post hoc</i> test a <i>p</i><0.050.