Erythroid population in bone marrow and spleen.

<p>Mice were given cadmium chloride dissolved in drinking water (50 ppm and 1000 ppm). Mice were sacrificed and their bone marrow and spleen cells were harvested. Isolated cells were double stained with anti-mouse CD71-PE and anti-mouse Ter119-APC and proportion of cells in different stages of erythropoiesis were determined. Four distinct stages of erythroid differentiation could be identified within an inverted ‘L’ shaped gate in the flow diagram. These include early proerythroblasts (CD71^highTer119^med), early basophilic erythroblast or erythroblasts A (Ter119^highCD71^highFSC^high), late basophilic polychromatic and orthochromatic erythroblasts or erythroblasts B (Ter119^highCD71^highFSC^low), and orthochromatic erythroblasts or erythroblasts C (Ter119^highCD71^lowFSC^low). Total erythroid population (panel A), and erythroid cells in different maturational stages in bone marrow (panel B) and spleen (panel C) after 5 weeks of cadmium exposure have been shown above. Each bar in the graph represents mean ± SEM of observations on 5–6 mice. *p<0.05 and ***p<0.005 for comparison of the groups (Student t-test).